Now growers will choose to use LED grow lights to fill light. Because the growth of plants can be affected by the control and adjustment of LED grow lights to achieve the effect that growers want, let’s look at the ways in which LED grow lights affect the growth and development of tissue culture plants?
Photoperiod regulation
LED grow light cycle is another important light environment factor that affects plant tissue culture. Affects the various developmental processes of plants, including rooting and stem growth, seed germination and flowering induction, and stoma formation and regulation. Under the red light of LED grow lights, the 16-hour photoperiod is most conducive to the formation of orchid protocorm callus. The LED grow light cycle is equal to or less than 12h to promote the differentiation of the bulbs of Lily Lily test-tube seedlings, and the formation rate reaches 100%. When the photoperiod is 8h, the fresh quality of the small bulbs reaches the maximum. The researchers found that the 8h photoperiod is beneficial to the sea The elongation of roots and stems of taro tissue culture seedlings and the increase of chlorophyll content are conducive to the increase of carbohydrate content and the widening of leaf stomata. The dry quality/fresh quality value of chrysanthemum was the largest under the 24-hour first cycle, and the number of leaves and dry quality accumulated the fastest. The potato tissue culture seedlings under the 16h LED grow light cycle are better under the combined red and blue light than the alternating red and blue light. The above shows that by regulating the light cycle of LED plants, the growth and development of tissue culture plants can be better affected.
Light intensity control
The intensity of LED grow light has an important effect on the formation of plant morphology and organs. Researchers found that 30 μmol/m²/s is beneficial to the elongation of stems and roots of hypnotic sleeping eggplant tissue culture seedlings. The leaf area and number of leaves at 30 and 60 μmol/m²/s are significantly higher than other light intensity treatments. Only at higher light intensity can the buds occur. Researchers' optimal light intensity for in vitro culture of chrysanthemum is 40 μmol/m²/s, under which the plant height and root length are the largest. The researchers found that it was 270μmol/m²/s. Under LED grow light intensity, the leaf area, leaf thickness and rooting rate of tissue cultured seedlings of Dolily Phalaenopsis were significantly higher than 450 μmol/m²/s. The rooting rate of Taro tissue cultured seedlings was highest under the light intensity of 30 μmol/m²/s, and the rooting rate decreased under the light intensity of 60 or 90 μmol/m²/s. The LED light intensity of 270μmol/m²/s is most conducive to the rooting of rose tissue culture seedlings, and the rooting rate decreases under the light intensity of 90 or 180μmol/m²/s. The above shows that different light intensities have different effects on the growth of various organs of tissue culture plants.
Light quality control
The callus formation and differentiation potential are different under different light quality. The callus irradiated under blue light has strong potential differentiation ability and the SOD activity is higher at this time. Researchers found that blue light significantly increased the SOD activity of grape test-tube plantlets, while red light significantly reduced its SOD activity. Under blue light culture, the number of POD isoenzyme bands at any stage of longan somatic embryo development is the most, and the enzyme activity is also relatively strong. Under red, green and white light, the POD of all stages of somatic embryo development is the same. The number and activity of zymogram bands vary to varying degrees. However, the number and activity of POD isozyme bands do not change much at all stages of somatic embryo development in the dark. This indicates that light has an effect on somatic embryogenesis of longan. The production and activity of POD isoenzymes at each stage of the phosphate have obvious effects. The above shows that different light qualities have different effects on the growth of plants. The regulation of light quality should be carried out according to the needs of plant species.
While LED grow lights supplement light for the growth and development of tissue culture plants, we must pay attention to the following issues.
Pay attention to the fill light time of LED grow lights
If the weather is fine, the plants can fill up the light for 2-3 hours in the morning and evening when the light is weak. If it is cloudy, rainy or hazy, plants can fill up the light all day. Moreover, there is a time limit for plants to supplement light. Plants have 14 hours of supplementary light a day, which is a very suitable time. They also need to rest at night and have time to breathe.
Pay attention to the distance between the LED grow light and the plant
LED grow lights will generate a certain amount of heat. If they get too close, they will burn the plants and cause some damage to the plants. However, if the distance between the LED grow light and the plant is too far, the light utilization efficiency will be greatly reduced, thereby weakening the light supplement effect. If the heat of LED grow lights is less than other grow lights, but there will be a certain amount of heat. At the same time, if the distance is too far, it will also affect the effect of the fill light.
Pay attention to the spectrum and power selection of LED grow lights
If you want to use LED grow lights to have a good effect, you must first choose the right LED grow light crops. Different crops have different requirements for contrast and light intensity, so pay attention to the spectrum and power of LED grow lights. For crops that need coloring and flowering, you can choose red and blue LED grow lights. For leafy vegetable crops, you can use full-spectrum LED grow lights. For plants that like light, you can choose high-power LED grow lights.
I hope that the above suggestions can provide you with reference value in planting. When using LED grow lights to fill light, you must also pay attention to related issues, so as to give plants an excellent growth state and environment.
